This field shows large and small pyramidal neurons and neuroglia. The intense staining of the cytoplasm of the pyramidal cells is mainly due to the basophilic Nissl bodies which are more easily seen in the dendrites (arrowheads). The nuclei of the large pyramidal cells are indicated (*).

The axons of these neurones are not stained with toluidine blue.

The small nuclei of neuroglia (arrows), most of which are oligodendrocytes are surrounded by a narrow rim of lightly stained cytoplasm.

Stain: Toluidine blue
Magnification: ×1000